Viral respiratory infections and the oropharyngeal bacterial microbiota in acutely wheezing children
Identifieur interne : 000D10 ( Main/Exploration ); précédent : 000D09; suivant : 000D11Viral respiratory infections and the oropharyngeal bacterial microbiota in acutely wheezing children
Auteurs : Leah Cuthbertson [Royaume-Uni] ; Stephen W. C. Oo [Australie] ; Michael J. Cox [Royaume-Uni] ; Siew-Kim Khoo [Australie] ; Des W. Cox [Australie] ; Glenys Chidlow [Australie] ; Kimberley Franks [Australie] ; Franciska Prastanti [Australie] ; Meredith L. Borland [Australie] ; James E. Gern [États-Unis] ; David W. Smith [Australie] ; Joelene A. Bizzintino [Australie] ; Ingrid A. Laing [Australie] ; Peter N. Le Souëf [Australie] ; Miriam F. Moffatt [Royaume-Uni] ; William O. C. Cookson [Royaume-Uni]Source :
- PLoS ONE [ 1932-6203 ] ; 2019.
Abstract
Acute viral wheeze in children is a major cause of hospitalisation and a major risk factor for the development of asthma. However, the role of the respiratory tract microbiome in the development of acute wheeze is unclear. To investigate whether severe wheezing episodes in children are associated with bacterial dysbiosis in the respiratory tract, oropharyngeal swabs were collected from 109 children with acute wheezing attending the only tertiary paediatric hospital in Perth, Australia. The bacterial community from these samples was explored using next generation sequencing and compared to samples from 75 non-wheezing controls. No significant difference in bacterial diversity was observed between samples from those with wheeze and healthy controls. Within the wheezing group, attendance at kindergarten or preschool was however, associated with increased bacterial diversity. Rhinovirus (RV) infection did not have a significant effect on bacterial community composition. A significant difference in bacterial richness was observed between children with RV-A and RV-C infection, however this is likely due to the differences in age group between the patient cohorts. The bacterial community within the oropharynx was found to be diverse and heterogeneous. Age and attendance at day care or kindergarten were important factors in driving bacterial diversity. However, wheeze and viral infection were not found to significantly relate to the bacterial community. Bacterial airway microbiome is highly variable in early life and its role in wheeze remains less clear than viral influences.
Url:
DOI: 10.1371/journal.pone.0223990
PubMed: 31622414
PubMed Central: 6797130
Affiliations:
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Bizzintino</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Telethon Kids Institute, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Laing, Ingrid A" sort="Laing, Ingrid A" uniqKey="Laing I" first="Ingrid A." last="Laing">Ingrid A. Laing</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Telethon Kids Institute, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Le Souef, Peter N" sort="Le Souef, Peter N" uniqKey="Le Souef P" first="Peter N." last="Le Souëf">Peter N. Le Souëf</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Telethon Kids Institute, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Moffatt, Miriam F" sort="Moffatt, Miriam F" uniqKey="Moffatt M" first="Miriam F." last="Moffatt">Miriam F. Moffatt</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>National Heart and Lung Institute, Imperial College, London, England, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>National Heart and Lung Institute, Imperial College, London, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation></author><author><name sortKey="Cookson, William O C" sort="Cookson, William O C" uniqKey="Cookson W" first="William O. C." last="Cookson">William O. C. 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C." last="Oo">Stephen W. C. Oo</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="2"><nlm:aff id="aff003"><addr-line>Respiratory Department, Perth Children’s Hospital, Perth, Western Australia</addr-line></nlm:aff><country>Australie</country><placeName><region type="state">Australie-Occidentale</region></placeName><wicri:cityArea>Respiratory Department, Perth Children’s Hospital, Perth</wicri:cityArea></affiliation></author><author><name sortKey="Cox, Michael J" sort="Cox, Michael J" uniqKey="Cox M" first="Michael J." last="Cox">Michael J. Cox</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>National Heart and Lung Institute, Imperial College, London, England, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>National Heart and Lung Institute, Imperial College, London, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation></author><author><name sortKey="Khoo, Siew Kim" sort="Khoo, Siew Kim" uniqKey="Khoo S" first="Siew-Kim" last="Khoo">Siew-Kim Khoo</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Telethon Kids Institute, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Cox, Des W" sort="Cox, Des W" uniqKey="Cox D" first="Des W." last="Cox">Des W. 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Borland</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff006"><addr-line>Emergency Department, Perth Children’s Hospital, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Emergency Department, Perth Children’s Hospital, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff007"><addr-line>Division of Emergency Medicine, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Emergency Medicine, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Gern, James E" sort="Gern, James E" uniqKey="Gern J" first="James E." last="Gern">James E. Gern</name><affiliation wicri:level="2"><nlm:aff id="aff008"><addr-line>Department of Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pediatrics, University of Wisconsin-Madison, Madison, Wisconsin</wicri:regionArea><placeName><region type="state">Wisconsin</region></placeName></affiliation></author><author><name sortKey="Smith, David W" sort="Smith, David W" uniqKey="Smith D" first="David W." last="Smith">David W. Smith</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff005"><addr-line>Department of Microbiology, PathWest Laboratory Medicine WA, QEII Medical Centre, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Microbiology, PathWest Laboratory Medicine WA, QEII Medical Centre, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff009"><addr-line>Medical School, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Medical School, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Bizzintino, Joelene A" sort="Bizzintino, Joelene A" uniqKey="Bizzintino J" first="Joelene A." last="Bizzintino">Joelene A. Bizzintino</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Telethon Kids Institute, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Laing, Ingrid A" sort="Laing, Ingrid A" uniqKey="Laing I" first="Ingrid A." last="Laing">Ingrid A. Laing</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Telethon Kids Institute, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Le Souef, Peter N" sort="Le Souef, Peter N" uniqKey="Le Souef P" first="Peter N." last="Le Souëf">Peter N. Le Souëf</name><affiliation wicri:level="1"><nlm:aff id="aff002"><addr-line>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Division of Paediatrics, Faculty of Health and Medical Sciences, University of Western Australia, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff004"><addr-line>Telethon Kids Institute, Perth, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Telethon Kids Institute, Perth</wicri:regionArea><wicri:noRegion>Perth</wicri:noRegion></affiliation></author><author><name sortKey="Moffatt, Miriam F" sort="Moffatt, Miriam F" uniqKey="Moffatt M" first="Miriam F." last="Moffatt">Miriam F. Moffatt</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>National Heart and Lung Institute, Imperial College, London, England, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>National Heart and Lung Institute, Imperial College, London, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation></author><author><name sortKey="Cookson, William O C" sort="Cookson, William O C" uniqKey="Cookson W" first="William O. C." last="Cookson">William O. C. Cookson</name><affiliation wicri:level="1"><nlm:aff id="aff001"><addr-line>National Heart and Lung Institute, Imperial College, London, England, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>National Heart and Lung Institute, Imperial College, London, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff010"><addr-line>Royal Brompton and Harefield NHS Foundation Trust, London, England, United Kingdom</addr-line></nlm:aff><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Royal Brompton and Harefield NHS Foundation Trust, London, England</wicri:regionArea><wicri:noRegion>England</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Acute viral wheeze in children is a major cause of hospitalisation and a major risk factor for the development of asthma. However, the role of the respiratory tract microbiome in the development of acute wheeze is unclear. To investigate whether severe wheezing episodes in children are associated with bacterial dysbiosis in the respiratory tract, oropharyngeal swabs were collected from 109 children with acute wheezing attending the only tertiary paediatric hospital in Perth, Australia. The bacterial community from these samples was explored using next generation sequencing and compared to samples from 75 non-wheezing controls. No significant difference in bacterial diversity was observed between samples from those with wheeze and healthy controls. Within the wheezing group, attendance at kindergarten or preschool was however, associated with increased bacterial diversity. Rhinovirus (RV) infection did not have a significant effect on bacterial community composition. A significant difference in bacterial richness was observed between children with RV-A and RV-C infection, however this is likely due to the differences in age group between the patient cohorts. The bacterial community within the oropharynx was found to be diverse and heterogeneous. Age and attendance at day care or kindergarten were important factors in driving bacterial diversity. However, wheeze and viral infection were not found to significantly relate to the bacterial community. Bacterial airway microbiome is highly variable in early life and its role in wheeze remains less clear than viral influences.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Goto, T" uniqKey="Goto T">T Goto</name></author><author><name sortKey="Tsugawa, Y" uniqKey="Tsugawa Y">Y Tsugawa</name></author><author><name sortKey="Mansbach, Jm" uniqKey="Mansbach J">JM Mansbach</name></author><author><name sortKey="Camargo, Ca" uniqKey="Camargo C">CA Camargo</name></author><author><name sortKey="Hasegawa, K" uniqKey="Hasegawa K">K Hasegawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bisgaard, H" uniqKey="Bisgaard H">H Bisgaard</name></author><author><name sortKey="Hermansen, Mn" uniqKey="Hermansen M">MN Hermansen</name></author><author><name sortKey="Buchvald, F" uniqKey="Buchvald F">F Buchvald</name></author><author><name sortKey="Loland, L" uniqKey="Loland L">L Loland</name></author><author><name 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C." last="Cookson">William O. C. Cookson</name><name sortKey="Cookson, William O C" sort="Cookson, William O C" uniqKey="Cookson W" first="William O. C." last="Cookson">William O. C. Cookson</name><name sortKey="Cox, Michael J" sort="Cox, Michael J" uniqKey="Cox M" first="Michael J." last="Cox">Michael J. Cox</name><name sortKey="Moffatt, Miriam F" sort="Moffatt, Miriam F" uniqKey="Moffatt M" first="Miriam F." last="Moffatt">Miriam F. Moffatt</name></country><country name="Australie"><noRegion><name sortKey="Oo, Stephen W C" sort="Oo, Stephen W C" uniqKey="Oo S" first="Stephen W. C." last="Oo">Stephen W. C. Oo</name></noRegion><name sortKey="Bizzintino, Joelene A" sort="Bizzintino, Joelene A" uniqKey="Bizzintino J" first="Joelene A." last="Bizzintino">Joelene A. Bizzintino</name><name sortKey="Bizzintino, Joelene A" sort="Bizzintino, Joelene A" uniqKey="Bizzintino J" first="Joelene A." last="Bizzintino">Joelene A. Bizzintino</name><name sortKey="Borland, Meredith L" sort="Borland, Meredith L" uniqKey="Borland M" first="Meredith L." last="Borland">Meredith L. Borland</name><name sortKey="Borland, Meredith L" sort="Borland, Meredith L" uniqKey="Borland M" first="Meredith L." last="Borland">Meredith L. Borland</name><name sortKey="Borland, Meredith L" sort="Borland, Meredith L" uniqKey="Borland M" first="Meredith L." last="Borland">Meredith L. Borland</name><name sortKey="Chidlow, Glenys" sort="Chidlow, Glenys" uniqKey="Chidlow G" first="Glenys" last="Chidlow">Glenys Chidlow</name><name sortKey="Cox, Des W" sort="Cox, Des W" uniqKey="Cox D" first="Des W." last="Cox">Des W. Cox</name><name sortKey="Franks, Kimberley" sort="Franks, Kimberley" uniqKey="Franks K" first="Kimberley" last="Franks">Kimberley Franks</name><name sortKey="Franks, Kimberley" sort="Franks, Kimberley" uniqKey="Franks K" first="Kimberley" last="Franks">Kimberley Franks</name><name sortKey="Khoo, Siew Kim" sort="Khoo, Siew Kim" uniqKey="Khoo S" first="Siew-Kim" last="Khoo">Siew-Kim Khoo</name><name sortKey="Khoo, Siew Kim" sort="Khoo, Siew Kim" uniqKey="Khoo S" first="Siew-Kim" last="Khoo">Siew-Kim Khoo</name><name sortKey="Laing, Ingrid A" sort="Laing, Ingrid A" uniqKey="Laing I" first="Ingrid A." last="Laing">Ingrid A. Laing</name><name sortKey="Le Souef, Peter N" sort="Le Souef, Peter N" uniqKey="Le Souef P" first="Peter N." last="Le Souëf">Peter N. Le Souëf</name><name sortKey="Le Souef, Peter N" sort="Le Souef, Peter N" uniqKey="Le Souef P" first="Peter N." last="Le Souëf">Peter N. Le Souëf</name><name sortKey="Oo, Stephen W C" sort="Oo, Stephen W C" uniqKey="Oo S" first="Stephen W. C." last="Oo">Stephen W. C. Oo</name><name sortKey="Prastanti, Franciska" sort="Prastanti, Franciska" uniqKey="Prastanti F" first="Franciska" last="Prastanti">Franciska Prastanti</name><name sortKey="Prastanti, Franciska" sort="Prastanti, Franciska" uniqKey="Prastanti F" first="Franciska" last="Prastanti">Franciska Prastanti</name><name sortKey="Smith, David W" sort="Smith, David W" uniqKey="Smith D" first="David W." last="Smith">David W. Smith</name><name sortKey="Smith, David W" sort="Smith, David W" uniqKey="Smith D" first="David W." last="Smith">David W. Smith</name><name sortKey="Smith, David W" sort="Smith, David W" uniqKey="Smith D" first="David W." last="Smith">David W. Smith</name></country><country name="États-Unis"><region name="Wisconsin"><name sortKey="Gern, James E" sort="Gern, James E" uniqKey="Gern J" first="James E." last="Gern">James E. Gern</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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